More attention should be paid to these patients for avoiding the fertilization failure. Patients with low percentage of morphologically normal sperm (<4%), low sperm concentration and motility, male primary infertility, female primary infertility, without oviduct obstruction, long female infertility years or/and couples primary infertility are at high risk of fertilization failure. After that, it was found by multivariate logistic regression analyses that many factors including percentage of morphologically normal sperm, sperm concentration, couples primary infertility and female infertility years were related with fertilization failure. Besides, higher total fertilization failure rate was found in couples with isolated teratozoospermia than in couples with normal percentage of morphologically normal sperm (15.0% vs 5.2%). The percentage of morphologically normal sperm (11.1 ± 5.8% vs 13.4 ± 5.3%), progressive motility (47.4 ± 10.5% vs 50.1 ± 8.6%), percentage of couple primary infertility (79.1% vs 44.3%), percentage of female primary infertility (69.1% vs 36.3%), percentage of male primary infertility (74.8% vs 41.7%), percentage of without oviduct obstruction patients (30.2% vs 13.6%) and percentage of couples primary infertility (79.1% vs 44.3%) of the fertilization failure patients were significantly different from those of the fertilized patients (P<0.05). The total fertilization failure rate of conventional IVF-ET rate was 5.7% (139/2 429). Risk factors were identified by univariate and multivariate logistic regression analyses. The total fertilization failure rate of 2 429 IVF cycles from January 2011 to May 2012 in the Reproductive Centre of the Women and Children Hospital of Jiangxi Province were retrospectively analyzed. To analyze the factors relating with fertilization failure in conventional IVF cycles. Such a pharmacological agent may provide a useful way of increasing the effectiveness of IUI or IVF over conventional IUI and IVF procedures. This raises the potential for future drug discovery to try to correct this defect by augmenting Ca²⁺ signalling such as Ca²⁺ store mobilisation or activating CatSper, as possible rational treatments for sperm dysfunction that may temporarily increase the capacity to interact with the egg. Interestingly, it appears that the potential exists to diagnose abnormalities in the Ca²⁺ channels that underlie sperm dysfunction. Ca²⁺ signalling plays a fundamental role in the regulation of sperm function. Thus, the limited success of ART in a proportion of male infertility cases is unsurprising. The best option to help couples with such male factor to achieve a pregnancy, is using Assisted Reproductive Technology (ART), without defining the underlying cause of sperm dysfunction or male-factor infertility in general at the cellular and molecular levels. Sperm dysfunction is the most common cause of male infertility. Sperm functions are an important factor for fertility/pregnancy to be achieved. These data demonstrate, for theįirst time, that a homogeneous live population of human hyperactivated spermatozoa, selected in vitro from patients with highly variable degrees of teratozoospermia, is comprised predominantly of cells with normal morphology Hyperactivated spermatozoa had a significantly higher mean percentage of normal heads and small acrosomes (P < 0.0001 and < 0.0001 respectively) and a significantly lower percentage of large and round heads, midpieces and tail defects Fifty-six patientĮjaculates were examined comprising a total morphological analysis of 1886 non-hyp spermatozoa and 1051 hyperactivated spermatozoa. These spermatozoa, visually identified onĪ tracking monitor, were individually removed with micromanipulation equipment using a 12 μm-diameter needle.
Specially developed software incorporated in the HST produced a whiteĬomputer-generated overlay for spermatozoa satisfying hyperactivation criteria. (LIN) ≤30% and straight-line velocity (VSL) ≤30 μm/s. (HST), sampling at 25 Hz, as curvilinear velocity (VCL) ≥70 μm/s, amplitude of lateral head displacement (ALH) ≥7 μm, linearity
Hyperactivation criteria were established by the Hobson Sperm Tracker The objective of this study was to compare the morphology of human spermatozoa undergoing hyperactivated motility in vitro with those that were non-hyperactivated (non-hyp).